These images span the length of Johnston's major portraiture years, beginning in 1895 with 23 shots of the first sitter in her studio, Frances Hodgson Burnett, and including a series of Supreme Court Justices which Johnston made in 1905. The sitters are divided into the following categories and finding guides are available according to these topics: Presidents, Cabinet members, Senators, House members, Supreme Court Justices, Diplomats, State Department employees, Women, Government Commissions, and Portraits (including authors, artists, poets, children -essentially all those who are not affiliated with the government either by direct appointment or marriage). The large format portraits are housed in Box 12.

Portraits of government groups such as the U.S. Mint employees (1889), Senate Military Affairs Committee (1891), Assistant Secretaries of the Treasury (1901), and the U.S. Delegates to the Pan-American Congress (1906); Views of government commissions including the Venezuelan Commission (1896), Samoan Commission (1899), First and Second Philippine Commissions (1899, 1900), Chinese High Commission, Presidential Commission to receive Prince Henry; Views related to the Presidential administration of William McKinley such as his Cabinet (1901), the second inauguration (1901), the Pan-American Exposition (including the last photograph of McKinley before he was assassinated at this even in 1901), and, later, a series of the unveiling ceremonies at the McKinley Memorial in Canton, Ohio (1907); Other ceremonial events include the unveiling of the Rochambeau Statue by President Theodore Roosevelt in Lafayette Square (1902), and laying the cornerstone for the Pan American Union Building (1908), also by President Roosevelt. Additional events include the Exchange of Warrents for the Philippines (1899), and the Opening of the 60th U.S. Congress (1907). For large format group portraits and government events refer to Box 13.

Cyanotype copies of Brady daguerreotypes of male sitters, many unidentified, which Johnston made from the originals in the War Department collections. There are identified portraits of Henry Clay, George Custis, John M. Clayton, William L. Dayton, and Winfield Scott.

Cyanotype copies of eighteen portraits in the War Department Collections. Six small head-and-shoulder views are included on each 8x10 inch negative (three in all). All the men are identified.

A variety of original texts which Johnston photographed (possibly for an article?) including the Articles of Confederation, the Oaths of Allegiance, the Treaty of Paris, Treaty and Seal of George I, Treaty of the War of 1812, C.A. Dana letter, Signature Treaty of the Mexican War, Acts of the 51st Congress, and John Quincy Adams' passport among other documents, some unidentified.

This series was taken by Johnston to illustrate Ida Tarbell's book, The Life of Abraham Lincoln [1903]. The images include a portrait of Abraham Lincoln and Tad (Meserve 39), Lincoln's chair and his Bible, documents and products with Lincoln labels. Most of the items were probably in the War Department collections.

This grouping has a series of photographs of the first telephone instruments which, Johnston explains on an index card, were made for Mrs. Bell. There are prints of the Francis E. Spinner Statue, a painting depicting Prince Edward of Wales at the Tomb of [George] Washington, and a group portrait of the survivors of the Johnstown Disaster.

Views of the imposing diplomatic residences of Washington, DC, many of which have been demolished. Most of these images were taken by Johnston for a series of articles that she authored entitled "The Foreign Legations of Washington, Parts 1-4" in Demorest's Family Magazine (April-July 1893). There are exterior and interior views of the Austrian, British, and French Embassies as well as the Brazilian, Chinese, Japanese, Korean, Mexican, and Russian Legations. The addresses of the buildings (included in the item-level guide) were provided by The Commission of Fine Arts in the 1970s.

The majority of these photographs showcase some of the more elaborate residences in turn-of-the-century Washington, DC. The impressive interiors of the John Wanamaker and William Whitney homes were the subject of articles by Johnston, again for Demorest's (July-December 1890) entitled "Some Homes Under the Administration." Additionally, there are views of the State Department and Department of Justice interiors, Senator Albert Beveridge's office, and the residences of Henry Brown, Charles Fairchild, Horace Gray, John Hay, Philander Knox, and photographs of Maytham Hall and Dorchester House, both in England. Maytham Hall was the estate of author Frances Hodgson Burnett, and Johnston took views of the gardens, grounds, and large house in 1906. There are two views of Burnett, author of The Secret Garden among other works, posing in her garden. There are many interior shots of Dorchester House, the American Embassy in London, which Johnston took in 1906.

Most of these portraits are printed from glass plate negatives which are 11x14 in. and larger. Included are views of Theodore Roosevelt (1902), a signed and mounted platinum print of Frances Folsom Cleveland, and impressive studio portraits of Julian Pauncefote, Wu T'ing Fang and his wife, Madame Wu. There is also a series of salt prints of illustrious men, many of the prints autographed by the sitters. The images are duplicates of the 8x10 in. portraits listed in the earlier boxes and appear to be a series that Johnston was preparing as a limited edition for sale.

Again, these views are printed from Johnston's 11x14 in. and larger glass plate negatives. This notable grouping included a mounted, platinum print of the Officers of the First National Congress of Mothers, February 1897. This organization was the forerunner of the Parent-Teacher Association, and the portrait is autographed by all the sitters. There is an interesting series of cyanotype proofs and platinum prints of Mrs. Cleveland and the Ladies of the Cabinet (#1250-1257). Both sittings (January, February 1897) are included and two of the mounted prints are signed by some of the women in attendance. Other items are group views of the first and second McKinley Cabinets; Signing of the Ratification of the Treaty with Spain (1898,1899); Opening Ceremonies of the 59th U.S. Congress (1905).