Register of the Russell Doolittle Biochemistry Laboratory Records
Mandeville Special Collections Library 0175S
UC, San Diego
La Jolla, CA 92093-0175
Phone: (858) 534-2533
Fax: (858) 534-5950
Email: spcoll@ucsd.edu
Repository Website: http://orpheus.ucsd.edu/speccoll/
© 1997
The Regents of the University of California. All rights reserved.
Register of the Russell Doolittle Biochemistry Laboratory Records, 1971-1985
MSS 0077
Mandeville Special Collections Library
GEISEL LIBRARY
UNIVERSITY OF CALIFORNIA, SAN DIEGO
La Jolla, CA 92093-0175
Contact Information:
- Mandeville Special Collections Library 0175S
- UC, San Diego
- La Jolla, CA 92093-0175
- Phone: (858) 534-2533
- Fax: (858) 534-5950
- Email: spcoll@ucsd.edu
- Repository Website: http://orpheus.ucsd.edu/speccoll/
© 1997 The Regents of the University of California. All rights reserved.
This file last updated: October 1997.
DESCRIPTIVE SUMMARY
Title: Russell Doolittle Biochemistry Laboratory Records,
Date (inclusive): 1971-1985
Collection number: MSS 0077
Extent: 33.00 linear feet (29 records cartons, 3 archives boxes
and 1 art bin item)
Repository:
Mandeville Special Collections Library, Geisel Library, UC, San Diego
Shelf Location: For current information on the location of these
materials, please consult the Library's online catalog.
Language:
English.
Preferred Citation
Russell Doolittle Biochemistry Laboratory Records, MSS 0077. Mandeville Special
Collections Library, UCSD.
ABSTRACT
The records (1971-1985) of Dr. Russell F. Doolittle's biochemistry laboratory at the
University of California, San Diego include notebooks related to the first determination
of the complete sequence of amino acids in the human fibrinogen molecule, paper files for
the amino acid sequences contained in the protein sequence data bank called NEWAT and an
amino acid sequencing machine.
The materials are arranged in three series: 1) NEWAT, 2) LABORATORY NOTEBOOKS and 3)
AMINO ACID SEQUENCER.
ADMINISTRATIVE HISTORY
Dr. Russell F. Doolittle, professor of chemistry at the University of California, San
Diego, heads a campus science laboratory that conducts research in the evolutionary and
structural aspects of proteins. In 1979, Doolittle's laboratory successfully analyzed the
structure of the amino acid sequence for the human fibrinogen molecule.
During that study, ten to twenty postdoctoral, graduate and undergraduate researchers
worked to pull apart and analyze the amino acid sequences in the alpha, beta and gamma
chains of fibrinogen. Doolittle adapted a peptide synthesizing machine to break apart
peptides and employed an amino acid analyzer to identify the sequences.
The peptide synthesizer, which was the second peptide synthesizer ever constructed, was
modeled on the prototype designed by Bruce Merrifield, then of Rockefeller University.
Merrifield was later awarded the Nobel Prize for the design of the machine. The UCSD
machine was constructed in 1964 at the instigation of Martin Kamen, a member of the
Chemistry faculty. Professor Kamen had seen Merrifield's machine and, on his return to
UCSD, asked Arthur Robinson, a graduate student in the Chemistry Department, to oversee
construction of the machine. Robinson journeyed back to New York to examine Merrifield's
machine more closely and then, after returning to UCSD, began constructing the UCSD
machine.
After a period of time, the machine was converted into a peptide sequencer. Instead of
building up peptides one amino acid at a time, it would take them apart one amino acid at
a time. In 1979, the machine was used to make the first determination of the complete
amino acid sequence for the human fibrinogen molecule, which is composed of 2,934 amino
acids.
Doolittle also constructed a protein sequence data bank called NEWAT. Sequences
identified in the published literature were entered into an electronic database and new
sequences were searched against known sequences. In 1983, Doolittle used the data bank to
link separate research efforts at the Center for Blood Research in Boston and the
National Cancer Institute in Washington, D. C. He related a known cancer gene to a gene
with a known function in human cells.
SCOPE AND CONTENT
The Russell Doolittle Biochemistry Laboratory Records document the activities of a UCSD
science laboratory engaged in research on the structure of the human fibrinogen molecule.
The materials are arranged in three series: 1) NEWAT, 2) LABORATORY NOTEBOOKS and 3)
AMINO ACID SEQUENCER.
SERIES 1: NEWAT
The NEWAT series contains journal articles, printouts and summary data reports for
individual amino acid sequences that were entered into the electronic data bank. The
series begins with a list that relates the folder title acronym to the name and the
journal citation for each amino acid sequence. The materials are arranged alphabetically
by acronym.
SERIES 2: LABORATORY NOTEBOOKS
The LABORATORY NOTEBOOKS series contains notebooks by individual postdoctoral, graduate
and undergraduate researchers who worked on the project. Also included are notebooks that
contain amino acid analysis charts and high performance liquid chromotography charts. The
notebooks are arranged by name of researcher, subproject and type of chart as follows:
2A) Watt, Ken; 2B) Bouma, H.; 2C) Takagi, T.; 2D) Miscellaneous Researchers; 2E) Electric
Birefringence; 2F) Peptide Synthesis; 2G) Analysis Charts; and 2H) High Performance
Liquid Chromotography Charts.
SERIES 3: AMINO ACID SEQUENCER
This is the machine adapted by laboratory staff to pull apart amino acids.
CONTAINER LIST
Box 2, Folder 1
ATPC1, -2, -3, -4, -5 to CHEY, .
Box 14, Folder 1
Book 1 - Part 1, .
Additional Note
Beta Chain CNBr / Lysozyme HSer Coupling, 10/01/76 -09/30/78.
Box 14, Folder 2
Book 1 - Part 2, .
Additional Note
Beta Chain CNBr / Lysozyme HSer Coupling, 01/01/77 -08/31/77.
Box 14, Folder 3
Book 2 - Part 1, .
Additional Note
H Beta 0 CN I, 11/01/76 -08/31/78.
Box 14, Folder 4
Book 2 - Part 2, .
Additional Note
H Beta 0 CN I, 11/01/76 -08/31/78.
Box 14, Folder 5
Book 2 - Part 3, .
Additional Note
H Beta 0 CN I, 11/01/76 -08/31/78.
Box 14, Folder 6
Book 3, .
Additional Note
H Beta 0 CN V, 11/01/76 -06/30/77.
Box 14, Folder 7
Book 4, .
Additional Note
H Beta 0 CN VII, 11/01/76 -07/31/77.
Box 14, Folder 8
Book 5 - Part 1, .
Additional Note
H Beta 0 CN II, 11/01/76 -09/30/78.
Box 14, Folder 9
Book 5 - Part 2, .
Additional Note
H Beta 0 CN II, 11/01/76 -09/30/78.
Box 14, Folder 10
Book 6 - Part 1, .
Additional Note
H Beta 0 CN VI, 11/01/76 -12/31/77.
Box 14, Folder 11
Book 6 - Part 2, .
Additional Note
H Beta 0 CN VI, 11/01/76 -12/31/77.
Box 14, Folder 12
Book 7, .
Additional Note
Sequence H Beta CN VId, 12/02/76 -03/31/78.
Box 14, Folder 13
Book 8, .
Additional Note
H Beta CN II, Glu Cleavage, 01/01/77 -11/30/77.
Box 14, Folder 14
Book 9, .
Additional Note
H Beta CN IV, 01/01/77 -12/31/77.
Box 15, Folder 1
Book 10, .
Additional Note
H Beta CN III, 01/01/77 -08/31/77.
Box 15, Folder 2
Book 11 - Part 1, .
Additional Note
Citraconylation, Triptic Digestion of Human 0 Beta Chain, 03/01/77 -06/01/77.
Box 15, Folder 3
Book 11 - Part 2, .
Additional Note
Citraconylation, Tryptic Digestion of Human 0 Beta Chain, 03/01/77 -06/30/77.
Box 15, Folder 4
Book 12, .
Additional Note
H Beta CN Vb, 03/01/77 -08/31/77.
Box 15, Folder 5
Book 13, .
Additional Note
CN VI C-2, CN VIf, H Beta CVI VI C-3, CN VI b-1, CN VIC-4, CN VI C-1, CN VIa, 03/01/77
-05/31/77.
Box 15, Folder 6
Book 14, .
Additional Note
H Beta CN IVb, 03/01/77 -07/31/77.
Box 15, Folder 7
Book 15 - Part 1, .
Additional Note
H Beta CN IIIa from Re-chromatog, 05/01/77 -10/31/77.
Box 15, Folder 8
Book 15 - Part 2, .
Additional Note
H Beta CN IIIa from Re-chromatog, 05/01/77 -10/31/77.
Box 15, Folder 9
Book 16, .
Additional Note
H Beta CN IIIb, CN IVa, 07/01/77 -08/31/77.
Box 15, Folder 10
Book 17 - Part 1, .
Additional Note
H Beta CN V TR, 07/01/77 -12/31/77.
Box 15, Folder 11
Book 17 - Part 2, .
Additional Note
H Beta CN V TR, 07/01/77 -12/31/77.
Box 15, Folder 12
Book 18, .
Additional Note
H Beta CN IV TR, Glu, 07/01/77 -09/31/77.
Box 15, Folder 13
Book 19 - Part 1, .
Additional Note
H Beta CN IVe, 07/01/77 -10/31/77.
Box 15, Folder 14
Book 19 - Part 2, .
Additional Note
H Beta CN IVe, 07/01/77 -10/31/77.
Box 15, Folder 15
Book 20 - Part 1, .
Additional Note
H Beta CN III CT and TR, 07/01/77 -08/31/77.
Box 15, Folder 16
Book 20 - Part 2, .
Additional Note
H Beta CN III CT and TR, 07/01/77 -08/31/77.
Box 15, Folder 17
Book 21, .
Additional Note
H Alpha 6d Ch, TR, 08/01/77 -06/30/79.
Box 15, Folder 18
Book 22, .
Additional Note
H Beta CN V Glu-, 10/01/77 -01/31/78.
Box 16, Folder 1
Book 23, .
Additional Note
H Beta CN III TR, 10/1/77 -10/31/77.
Box 16, Folder 2
Book 24 - Part 1, .
Additional Note
H Beta CT, 11/01/77 -01/31/78.
Box 16, Folder 3
Book 24 - Part 2, .
Additional Note
H Beta CT, 11/01/77 -03/31/78.
Box 16, Folder 4
Book 25, .
Additional Note
H Beta CN Ne CT, and Sequence, 12/01/77 -12/31/77.
Box 16, Folder 5
Book 26 - Part 1, .
Additional Note
H Beta Glu, 12/01/77 -2/28/78.
Box 16, Folder 6
Book 26 - Part 2, .
Additional Note
H Beta Glu, 12/01/77 -2/28/78.
Box 16, Folder 7
Book 26 - Part 3, .
Additional Note
H Beta Glu, 12/01/77 -2/28/78.
Box 16, Folder 8
Book 27 - Part 1, .
Additional Note
H Beta CN II Glu EDC Sequence, 01/01/78 -01/31/78.
Box 16, Folder 9
Book 27 - Part 2, .
Additional Note
H Beta CN II Glu EDC Sequence, 01/01/78 -01/31/78.
Box 16, Folder 10
Book 28 - Part 1, .
Additional Note
Alpha Chain CNI Sequence Summary, H Beta Glu Sequence, 02/01/78 -05/31/78.
Box 16, Folder 11
Book 28 - Part 2, .
Additional Note
Alpha Chain CNI Sequence Summary, H Beta Glu Sequence, 02/01/78 -05/31/78.
Box 16, Folder 12
Book 29, .
Additional Note
H Beta CNI GEE DITC Bends, 09/01/78 -09/31/78.
Box 16, Folder 13
Book 30 - Part 1, .
Additional Note
H Alpha CN1 PL1 SP1 Ch, 10/01/78 -03/31/79.
Box 16, Folder 14
Book 30 - Part 2, .
Additional Note
H Alpha CN1 PL1 SP1 Ch, 10/01/78 -03/31/79.
Box 16, Folder 15
Book 31 - Part 1, .
Additional Note
H Alpha CNI SPI Ch 2nd, 11/01/78 -02/28/79.
Box 16, Folder 16
Book 31 - Part 2, .
Additional Note
H Alpha CNI SPI Ch 2nd, 11/01/78 -02/28/79.
Box 16, Folder 17
Book 32, .
Additional Note
PLI SP2 Ch to SGSFRP...E, ENI SP Pool 2, 01/01/79 -02/28/79.
Box 16, Folder 18
Book 33, .
Additional Note
H Alpha CN1 SP2 Ch SP2dnp to Ng, 02/01/79 -03/31/79.
Box 16, Folder 19
Book 34, .
Additional Note
H Alpha CN1 SP1 Ch, 02/01/79 -03/31/79.
Box 16, Folder 20
Book 35 - Part 1, .
Additional Note
H Alpha Chain, 04/01/79 -06/30/79.
Box 17, Folder 1
Book 35 - Part 2, .
Additional Note
H Alpha Chain, 04/01/79 -06/30/79.
Box 17, Folder 2
Book 35 - Part 3, .
Additional Note
H Alpha Chain, 04/01/79 -06/30/79.
Box 17, Folder 3
Book 36 - Part 1, .
Additional Note
H Alpha, Glu Digest Continuation of 1/78 Prep (70mg), 05/01/79 -05/31/79.
Box 17, Folder 4
Book 36 - Part 2, .
Additional Note
H Alpha, Glu Digest Continuation of 1/78 Prep (70mg), 05/01/79 -05/31/79.
Box 17, Folder 5
Book 37 - Part 1, .
Additional Note
H Alpha Glu (sequence) 71mg prep, 05/01/79 -06/30/79.
Box 17, Folder 6
Book 37 - Part 2, .
Additional Note
H Alpha Glu (sequence) 71mg prep, 05/01/79 -06/30/79.
Box 17, Folder 7
Book 38 - Part 1, .
Additional Note
H Alpha -Subscript 2 -SP 72 6 mg G50, 06/01/79 -06/30/79.
Box 17, Folder 8
Book 38 - Part 2, .
Additional Note
H Alpha -Subscript 2 -SP 72 6mg G50, 06/01/79 -06/30/79.
Box 17, Folder 9
Book 38 - Part 3, .
Additional Note
H Alpha -Subscript 2 -SP 72 6mg G50, 06/01/79 -06/30/79.
Box 17, Folder 10
Book 38 - Part 4, .
Additional Note
H Alpha -Subscript 2 -SP 72 6 mg G50, 06/01/79 -06/30/79.
Box 17, Folder 11
Book 39, .
Additional Note
Ch H Alpha CNI PLII, 03/01/78 -07/31/78.
Box 17, Folder 12
Book 40 - Part 1, .
Additional Note
H Alpha -Subscript 2 -CNI Ch-, 03/01/78 -03/31/78.
Box 17, Folder 13
Book 40 - Part 2, .
Additional Note
H Alpha -Subscript 2 -CNI Ch-, 03/01/78 -03/31/78.
Box 17, Folder 14
Book 40 - Part 3, .
Additional Note
H Alpha -Subscript 2 -CNI Ch-, 03/01/78 -03/31/78.
Box 17, Folder 15
Book 41 - Part 1, .
Additional Note
H Alpha CNI Ch Sequence, 04/01/78 -08/31/78.
Box 17, Folder 16
Book 41 - Part 2, .
Additional Note
H Alpha CNI Ch Sequence, 04/01/78 -08/31/78.
Box 17, Folder 17
Book 41 - Part 3, .
Additional Note
H Alpha CNI Ch Sequence, 04/01/78 -08/31/78.
Box 17, Folder 18
Book 42, .
Additional Note
H Alpha, CNI Tryptophan, 04/01/78 -06/30/78.
Box 17, Folder 19
Book 43 - Part 1, .
Additional Note
H Beta Chain Trypson, 04/01/78 -05/31/78.
Box 17, Folder 20
Book 43 - Part 2, .
Additional Note
H Beta Chain Trypson, 04/01/78 -05/31/78.
Box 17, Folder 21
Book 44, .
Additional Note
Frag. D, CN III C, TR, 05/01/78 -07/31/78.
Box 18, Folder 1
Book 45 - Part 1, .
Additional Note
H Beta (CN Br), HD Beta Tryptic, 05/01/78 -09/30/78.
Box 18, Folder 2
Book 45 - Part 2, .
Additional Note
H Beta (CN Br), HD Beta Tryptic, 05/01/78 -09/30/78.
Box 18, Folder 3
Book 45 - Part 3, .
Additional Note
H Beta (CN Br), HD Beta Tryptic, 05/01/78 -09/30/78.
Box 18, Folder 4
Book 46, .
Additional Note
H Alpha CNI PLI Ch, 06/01/78 -06/30/78.
Box 18, Folder 5
Book 47, .
Additional Note
H Alpha CNI PLI Ch (EDC), 04/01/78 -08/31/78.
Box 18, Folder 6
Book 48, .
Additional Note
H Alpha CNI PL2, Ch2, Ch1, 07/01/78 -07/31/78.
Box 18, Folder 7
Book 49, .
Additional Note
II H Alpha CNI Trytophan, 08/01/78 -03/31/78.
Box 18, Folder 8
Book 50 - Part 1, .
Additional Note
H Alpha CN1 PL1 Ch 2hrs, 37 degrees 1/50 ration, partially GEE, 08/01/78 -01/31/79.
Box 18, Folder 9
Book 50 - Part 2, .
Additional Note
H Alpha CN1 PL1 Ch 2hrs, 37 degrees 1/50 ratio, partially GEE, 08/01/78 -01/31/79.
Box 18, Folder 10
Book 50 - Part 3, .
Additional Note
H Alpha CN1 PL1 Ch 2hrs, 37 degrees 1/50 ratio, partially GEE, 08/01/78 -01/31/79.
Box 18, Folder 11
Book 1 - Part 1, .
Additional Note
I. Human Fibrinogen; Gamma Chain; Citraconyl -Trypsin, G-50; Peaks V, VI, VII, VIII,
09/01/75 -03/31/76.
Box 18, Folder 12
Book 1 - Part 2, .
Additional Note
I. Human Fibrinogen; Gamma Chain; Citraconyl -Trypsin, G-50; Peaks V, VI, VII, VIII,
09/01/75 -03/31/76.
Box 18, Folder 13
Book 1 - Part 3, .
Additional Note
I. Human Fibrinogen; Gamma Chain; Citraconyl -Trypsin, G-50; Peaks V, VI, VII, VIII,
09/01/75 -03/31/76.
Box 18, Folder 14
Book 2 - Part 1, .
Additional Note
Human Fibrinogen; Gamma Chain; Citraconyl -Trypsin, G-50; Peaks II, III, IV, 09/01/75
-12/31/76.
Box 18, Folder 15
Book 2 - Part 2, .
Additional Note
Human Fibrinogen; Gamma Chain; Citraconyl -Trypsin, G-50; Peaks II, III, IV, 09/01/75
-12/31/76.
Box 18, Folder 16
Book 2 - Part 3, .
Additional Note
Human Fibrinogen; Gamma Chain; Citrconyl -Trypsin, G-50; Peaks II, III, IV, 09/01/75
-12/31/76.
Box 18, Folder 17
Book 3, .
Additional Note
Miscellaneous: Anti-human Fibrinogen; 4, 4'-diaminobenzil; TATG synthesis,
09/01/75-09/30/76.
Box 18, Folder 18
Book 4 - Part 1, .
Additional Note
Human Fibrinogen; Gamma Chain; Citraconyl -Trypsin, G-50; Peaks II, III, VIII, 01/01/76
-06/30/76.
Box 19, Folder 1
Book 4 - Part 2, .
Additional Note
Human Fibrinogen; Gamma Chain; Citraconyl-Trypsin, G-50; Peaks II, III, VIII, 01/01/76
-06/30/76.
Box 19, Folder 2
Book 4 - Part 3, .
Additional Note
Human Fibrinogen; Gamma Chain; Citraconyl-Trypsin, G-50; Peaks II, III, VIII, 01/01/76
-06/30/76.
Box 19, Folder 3
Book 5, .
Additional Note
Human Fibrinogen; Gamma Chain; Citraconyl-Trypsin, G-50; Peaks V and VI, 01/01/76
-08/31/76.
Box 19, Folder 4
Book 6 - Part 1, .
Additional Note
Human Fibrinogen; Gamma Chain; Citraconyl-Tripsin; G-50; Peak II; Deblocked, Trypsin,
G-25, 01/01/76 -03/31/76.
Box 19, Folder 5
Book 6 - Part 2, .
Additional Note
Human Fibrinogen; Gamma Chain; Citraconyl-Tripsin; G-50; Peak II; Deblocked, Trypsin,
G-25, 01/01/76 -03/31/76.
Box 19, Folder 6
Book 7 - Part 1, .
Additional Note
Human Fibrinogen; Gamma Chain; Succinylated, Aminoethylated; Trypsin, G-25; Peaks 1, 4,
5, and 6, 10/01/76 -01/31/77.
Box 19, Folder 7
Book 7 - Part 2, .
Additional Note
Human Fibrinogen, Gamma Chain; Succinylated, Aminoethylated; Trypsin, G-25; Peaks 1, 4,
5, and 6, 10/01/76 -01/31/77.
Box 19, Folder 8
Book 8 - Part 1, .
Additional Note
Human Fibrinogen; Gamma Chain; Succinylated, 14 C-aminoethylated; Trypsin, G-25; Peaks 1,
4, 5, and 6 (synthesis: 14 C-bromoethylamine), 12/01/76 -08/31/77.
Box 19, Folder 9
Book 8 - Part 2, .
Additional Note
Human Fibrinogen; Gamma Chain; Succinylated, 14 C-aminoethylated; Trypsin, G-25; Peaks 1,
4, 5, and 6 (synthesis: 14 C-bromoethylamine), 12/01/76 -08/31/77.
Box 19, Folder 10
Book 9 - Part 1, .
Additional Note
Fibrinogen Sulfitolysis; S-sulfo Human Fibrinogen Separations; Gamma Chain Modifications
(aminoethylation, cyclohexanedione, tryptic cleavages), 08/01/76 -11/31/76.
Box 19, Folder 11
Book 9 - Part 2, .
Additional Note
Fibrinogen Sulfitolysis; S-sulfo Human Fibrinogen Separations; Gamma Chain Modifications
(aminoethylation, cyclohexanedione, tryptic cleavages), 08/01/76 -11/31/76.
Box 19, Folder 12
Book 10 - Part 1, .
Additional Note
HG, CT2 (HG, CT2, AT3) (HG, CT2, AT4 and AT5); HG CT5; HG CT6; HG CT7, 03/01/77
-04/30/77.
Box 19, Folder 13
Book 10 - Part 2, .
Additional Note
HG, CT2 (HG, CT2, AT3) (HG, CT2, AT4 and AT5); HG CT5; HG CT6; HG CT7, 03/01/77
-04/30/77.
Box 19, Folder 14
Book 11 - Part 1, .
Additional Note
HG, CT 3B; TRa-2; TRb-2, 04/01/77 -06/30/77.
Box 19, Folder 15
Book 11 - Part 2, .
Additional Note
HG, CT 3B; TRa-2; TRb-2, 04/01/77 -06/30/77.
Box 19, Folder 16
Book 12 - Part 1, .
Additional Note
Plasmin -0ib DE-52; CNBr-G-100 Columns; TR-G-25 Columns, 08/01/77 -01/31/78.
Box 19, Folder 17
Book 12 - Part 2, .
Additional Note
Plasmin -0ib DE-52; CNBr-G-100 Columns; TR-G-25 Columns, 08/01/77 -01/31/78.
Box 19, Folder 18
Book 13 - Part 1, .
Additional Note
HF, PLD, CN III B; HF, PLD, CN III C, 12/01/77 -12/31/77.
Box 20, Folder 1
Book 13 - Part 2, .
Additional Note
HF, PLD, CN III B; HF, PLD, CN III C, 12/01/77 -12/31/77.
Box 20, Folder 2
Book 14, .
Additional Note
HF, PLD, CN II; HF, PLD, CN III, 02/01/78 -05/31/78.
Box 20, Folder 3
Book 15 - Part 1, .
Additional Note
G -37 1/2; HF, PLD, CN I, TRA-2; ... A-3; ... A-4, 12/01/77 -01/31/78.
Box 20, Folder 4
Book 15 - Part 2, .
Additional Note
G -37 1/2; HF, PLD, CN I, TRA-2; ... A-3; ... A-4, 12/01/77 -01/31/78.
Box 20, Folder 5
Book 16 - Part 1, .
Additional Note
HF, PLD, CN I, TRA; HF, PLD, CN I, TRB, 12/01/77 -03/31/78.
Box 20, Folder 6
Book 16 - Part 2, .
Additional Note
HF, PLD, CN I, TRA; HF, PLD, CN I, TRB, 12/01/77 -03/31/78.
Box 20, Folder 7
Book 16 - Part 3, .
Additional Note
HF, PLD, CN I, TRA; HF, PHD, CN I, TRB, 09/01/77 -11/30/77.
Box 20, Folder 8
Book 16 - Part 4, .
Additional Note
HF, PLD, CN I, TRA; HF, PLD, CN I, TR B, 09/01/77 -11/30/77.
Box 20, Folder 9
Book 17 - Part 1, .
Additional Note
Frag D; CN I, TR pH 7.0; Peak B-D, 03/01/78 -06/30/78.
Box 20, Folder 10
Book 17 - Part 2, .
Additional Note
Frag D; CN I, TR pH 7.0; Peak B-D, 03/01/78 -06/30/78.
Box 20, Folder 11
Book 18, .
Additional Note
Tryptic-digest; Human Fibrinogen; Gamma Chain, 06/01/78 -07/31/78.
Box 20, Folder 12
Book 19, .
Additional Note
Fragment D; CN I, TRA; pH 7.0 Trypsin; G-25, 04/01/78 -07/31/78.
Box 20, Folder 13
Book 1 - Part 1, .
Additional Note
H Gamma CT Pools I, II, III, DNS; CT I, II, III TATG (electrophoresis, CNBr), 11/01/73
-07/31/74.
Box 20, Folder 14
Book 1 - Part 2, .
Additional Note
H Gamma CT Pools I, II, III, DNS; CT I, II, III, TATG (electrophoresis, CNBr), 11/01/73
-07/31/74.
Box 20, Folder 15
Book 2 - Part 1, .
Additional Note
H Gamma CT IV -VIII (gel filtration, paper el., ion exchange -Aminex A5), 11/01/73
-07/31/74.
Box 20, Folder 16
Book 2 - Part 2, .
Additional Note
H Gamma CT IV -VIII (gel filtration, paper el., ion exchange -Aminex A5), 11/01/73
-07/31/74.
Box 20, Folder 17
Book 2 - Part 3, .
Additional Note
H Gamma CT IV -VIII (gel filtration, paper el., ion exchange -Aminex A5), 11/01/73
-07/31/74.
Box 20, Folder 18
Book 3 - Part 1, .
Additional Note
H Gamma CNB R and A; Trypsin I -IV (gel filtration, paper el.), 05/01/74 -08/31/74.
Box 21, Folder 1
Book 3 - Part 2, .
Additional Note
H Gamma CNB R and A; Trypsin I -IV (gel filtration, paper el.), 05/01/74 -08/31/74.
Box 21, Folder 2
Book 4, .
Additional Note
H Gamma chain; AAA 24, 48,72 hr.; Tryptophan Det with Methane Sulfonic Acid; H Gamma CNI
AAA 3/75, 07,01/73 -12/31/73.
Box 21, Folder 3
Book 5 - Part 1, .
Additional Note
H Gamma CNI Chymotrypsin Gel Filtration G-25 Paper El, 09/01/74 -11/30/74.
Box 21, Folder 4
Book 5 - Part 2, .
Additional Note
H Gamma CNI Chymotrypsin Gel Filtration G-25 Paper El, 09/01/74 -11/30/74.
Box 21, Folder 5
Book 6, .
Additional Note
H Gamma CNI Pepsin Gel Filtration G-25 Paper El, 10/01/74 -11/30/74.
Box 21, Folder 6
Book 7 - Part 1, .
Additional Note
H Gamma CNBr I, II, III B; AAA; Sequence; Trypsin; CN 5, 6 Purification Enzyme Digestion,
06/01/73 -02/28/74.
Box 21, Folder 7
Book 7 - Part 2, .
Additional Note
H Gamma CNBr I, II, III B; AAA; Sequence; Trypsin; CN 5, 6 Purification Enzyme Digestion,
06/01/73 -02/28/74.
Box 21, Folder 8
Book 7 - Part 3, .
Additional Note
H Gamma CNBr I, II, III B; AAA; Sequence; Trypsin; CN 5, 6 Purification Enzyme Digestion,
06/01/73 -02/28/74.
Box 21, Folder 9
Book 8, .
Additional Note
Gamma CN III, IV, V, 09/01/73 -10/31/74.
Box 21, Folder 10
Book 9 - Part 1, 1 of 2, .
Box 21, Folder 11
Book 9 - Part 1, 2 of 2, .
Box 21, Folder 12
Book 9 - Part 2, 1 of 2, .
Box 21, Folder 13
Book 9 - Part 2, 2 of 2, .
Box 21, Folder 14
Book 10, Collagenase Digestion of H0, .
Additional Note
3/74 -4/74.
Box 21, Folder 15
Book 11 - Part 1, 1 of 2 -- Plasmin Digestion of, .
Additional Note
4/74 -5/74.
Box 21, Folder 16
Book 11 - Part 1, 2 of 2 -- Plasmin Digestion of, .
Additional Note
4/74 -5/74.
Box 21, Folder 17
Book 11 - Part 2, 1 of 2 -- Plasmin Digestion of H0 90' 21hr, .
Additional Note
4/74 -5/74.
Box 21, Folder 18
Book 11 - Part 2, 2 of 2 -- Plasmin Digestion of H0 90' 21 hr, .
Additional Note
4/74 -5/74.
Box 21, Folder 19
Book 12 - Part 1 -- HD, CNBr G-100 Purification, Enzyme Digestions, .
Additional Note
1/75 -3/75.
Box 22, Folder 1
Book 12 - Part 2, .
Additional Note
01/75 -03/75.
Box 22, Folder 2
Book 13, .
Additional Note
Separation of D and E on Biogel A 1.5 M, 4 m Guanidine, 02/74 -07/74.
Box 22, Folder 3
Book 14, .
Additional Note
HDII: Separation, R and A, Purification, Enzyme Digestion, 08/74 -12/74.
Box 22, Folder 4
Book 15 - Part 1, .
Additional Note
HE R and A --G-25, Paper El, 07/74.
Box 22, Folder 6
Book 16 - Part 1, .
Additional Note
Gel Filtration of D and E, HE CNBr --G-50, Paper El of Peptides, 05/74-07/74.
Box 22, Folder 7
Book 16 - Part 2, .
Additional Note
05/74 -07/74.
Box 22, Folder 8
Book 17 - Part 1, .
Additional Note
HE CNBr (S-S) in E, Purification of CNBr Peptides, 08/74 -01/75.
Box 22, Folder 9
Book 17 - Part 2, .
Additional Note
08/74 -01/75.
Box 22, Folder 10
Book 18 - Part 1, .
Additional Note
FIIB-2, FIIB-3, FIIB-4, FIIB-5, D-D3, 02/74 -02/75.
Box 22, Folder 11
Book 18 - Part 2, .
Additional Note
02/74 -01/75.
Box 22, Folder 12
Book 19 - Part 1, .
Additional Note
CNBr Digestion of H Fibrin, 10/74 -02/75.
Box 22, Folder 13
Book 19 - Part 2, .
Additional Note
10/74 -02/75.
Box 22, Folder 14
Book 20 - Part 1, .
Additional Note
TATG of H Fragments D, E, 02/74.
Box 22, Folder 15
Book 20 - Part 2, .
Additional Note
TATG of H Fragment D, E, 02/74.
Box 22, Folder 16
Book 21, .
Additional Note
H0 CNBR, H Gamma CNBr, 02/74 -5/74.
Box 22, Folder 17
Book 22, .
Additional Note
H 14C GEE Labelled Gamma Chain, Fragments from Plasmin Digestion of H GEE Fibrins, 02/74
-05/74.
Box 22, Folder 18
Book 23, .
Additional Note
TATG of H Fragment D, E (see envelope), 05/74 -8/74.
Box 22, Folder 19
Book 24, .
Additional Note
Human Gamma G-50, Purfication of Pools 4.5 and 6, Enzyme Digestion of Cyanogen Bromide
Pools, 12/74 -02/75.
Box 23, Folder 1
Book 25 - Part 1, .
Additional Note
Beta chain CNBr, G-100 10% HAc Purfication, 08/74 -12/75.
Box 23, Folder 2
Book 25 - Part 2, .
Additional Note
08/75 -12/75.
Box 23, Folder 3
Book 26 - Part 1, .
Additional Note
Separation of H Gamma, Beta Chains CM52, Beta Chain CNBr Peptides AAA, 03/74.
Box 23, Folder 5
Book 27, .
Additional Note
Bov Gamma CNBr A,B,C, AAA (Bovine).
Box 23, Folder 6
Book 28, .
Additional Note
Oxidized Bov Gamma Chain and Its CNBr Fragments, Separation Biogel 1.5 Purification by
Paper El, 01/74 -02/74.
Box 23, Folder 7
Book 29, .
Additional Note
H Fragment A from Plasmin, digest 10', 90', 15 hrs, Enzyme Digestion, 05/72.
Box 23, Folder 8
Book A - Part 1, .
Additional Note
HF XIII: H "X" Trypsin ( Thermolysin, Chymotrypsin ); PCAase, Pronase, CPase; HX for
TATG; P -Ser; Pepsin; Platelet "X", 12/01/72 -10/31/73.
Box 23, Folder 9
Book A - Part 2, .
Additional Note
HF XIII: H "X" Trypsin ( Thermolysin, Chymotrypsin ); PCAase, Pronase, CPase; HX for
TATG; P -Ser; Pepsin; Platetet "X", 12/01/72 -10/31/73.
Box 23, Folder 10
Book A - Part 3, .
Additional Note
HF XIII: H "X" Trypsin ( Thermolysin, Chymotrypsin ); PCAase, Pronase, CPase; HX for
TATG; P -Ser; Pepsin; Platetet "X".
Box 23, Folder 11
Book A - Part 4, .
Additional Note
HF XIII: H "X" Trypsin ( Thermolysin, Chymotrypsin ); PCAase, Pronase, CPase; HX for
TATG; P -Ser; Pepsin; Platetet "X", 12/01/72 -10/31/73.
Box 23, Folder 12
Book B - Part 1, .
Additional Note
Preparation of BF XIII, HF XIII (Biogel A1.5, Platelet F XIII, DE-52 SOS gels);
Activation of F XIII; Trypsin Dig. of 14C GEE F XIII; Sulfitolysis of F XIII *;
Separation of A and B Chains of F XIII *, 09/01/72 -09/30/73.
Box 23, Folder 13
Book B - Part 2, .
Additional Note
Preparation of BF XIII, HF XIII (Biogel A1.5, Platelet F XIII, DE-52 SOS gels);
Activation of F XIII; Trypsin Dig. of 14C GEE F XIII; Sulfitolysis of F XIII *;
Separation of A and B Chains of F XIII *, 09/01/72 -09/30/73.
Box 23, Folder 14
Book C - Part 1, .
Additional Note
TATG: H, B FXIII, FXIII *; ClG; Platelet F XIII *; Seattle F XIII *, 11/01/72 -11/31/73.
Box 23, Folder 15
Book C - Part 2, .
Additional Note
TATG: H, B, F XIII, F XIII *; ClG; Platelet F XIII; Seattle F XIII *, 11/01/72 -11/31/73.
Box 23, Folder 16
Book C - Part 3, .
Additional Note
TATG: H, B, F XIII, F XIII *; ClG; Platelet F XIII *; Seattle F XIII *, 11/01/72
-11/31/73.
Box 23, Folder 17
Book D - Part 1, .
Additional Note
Bov P "X" F XIII; Preparation; Trypsin Digestion; Chymotrypsin; PCA to Th; Trypsin to Th,
10/01/72 -10/31/73.
Box 23, Folder 18
Book D - Part 2, .
Additional Note
Bov P "X" F XIII; Preparation; Trypsin Digestion; Chymotrypsin; PCA to Th; Trypsin to Th,
10/01/72 -10/31/73.
Box 23, Folder 19
Book E, .
Additional Note
Bov Gamma ( plasmin, trypsin SDS gels ); GEE B Gamma -Plasmin; B 0 plasmin, 08/01/72
-03/31/73.
Miscellaneous Researchers
Box 24, Folder 1
Friezner, S - Book 1 - Part 1, .
Additional Note
H Gamma CNI Trypsin, 06/75.
Box 24, Folder 2
Friezner, S - Book 1 - Part 2, .
Box 24, Folder 3
Lin, Y - Book 1, .
Additional Note
H 14C GEE Alpha Electrophoresis; T-boc Reactions T-boc-EA, 02/01/71 -05/31/71.
Box 24, Folder 4
Lin, Y - Book 2 - Part 1, .
Additional Note
Synthesis of HFpA; A-D-S-G-E-G-D-F-A-A-0; Preparation of Bovine Insulin; A and B Chains,
02/01/71 -05/31/74.
Box 24, Folder 5
Lin, Y - Book 2 - Part 2, .
Additional Note
Synthesis of HFpA; A-D-S-G-E-G-D-F-A-A-0; Preparation of Bovine Insulin; A and B Chains,
02/01/71 -05/31/74.
Box 24, Folder 6
Maffly, B. and Goldbaum, D - HPLC, .
Additional Note
04/76 -01/78.
Box 24, Folder 7
Cottrell, B. - Book 1 - Part 1, 8/73 - 11/73, .
Box 24, Folder 8
Cottrell, B. - Book 1 - Part 2, 8/73 - 11/73, .
Box 24, Folder 9
Cottrell, B. - Book 2 - Part 1, 1973, .
Additional Note
Photographs, computer programs and basic user manual.
Box 24, Folder 10
Cottrell, B. - Book 2 - Part 2, 4/74 - 6/74, .
Box 24, Folder 11
Cottrell, B. - Book 2 - Part 3, 4/74 - 6/74, .
Box 24, Folder 12
Cottrell, B. - Book 3 - Part 1, 6/74 - 7/74, .
Box 24, Folder 13
Cottrell, B. - Book 3 - Part 2, 6/74 - 7/74, .
Box 24, Folder 14
Cottrell, B. - Book 4 - Part 1, 8/74 - 3/75, .
Box 24, Folder 15
Cottrell, B. - Book 4 - Part 2, 8/74 - 7/75, .
Box 24, Folder 16
Cottrell, B. - Book 5 - Part 1, 3/75 - 5/75, .
Box 24, Folder 17
Cottrell, B. - Book 5 - Part 2, 3/75 - 5/75, .
Box 24, Folder 18
Cottrell, B. - Computer Graphs B/C, .
Box 25, Folder 1
Cottrell, B. - Computation Book, Oct 22, 1973, .
Box 25, Folder 2
Cottrell, B. - Computer Graphs, Upsilon, Theta - Part 1, .
Box 25, Folder 3
Cottrell, B. - Computer Graphs, Upsilon, Theta - Part 2, .
Box 25, Folder 4
Cottrell, B. - Data Output. Elbra - Part 1, .
Box 25, Folder 5
Cottrell, B. - Data Output. Elbra - Part 2, .
Box 25, Folder 6
Cottrell, B. - Log Book, .
Additional Note
Photographs.
Box 25, Folder 7
Cottrell, B. - Log Book, .
Additional Note
Photographs -Enlarged (part II).
Box 25, Folder 8
Troll, M. - Book 1, .
Additional Note
System logic of the laser birefringence machine, reprints, technical information,
equipment specifications (part 1).
Box 25, Folder 9
Troll, M. - Book 1, .
Additional Note
System logic of laser birefringence machine, reprints, technical information, equipment
specifications (part II).
Box 25, Folder 10
BM BIOCHEMICA - Vol. 2 Number 3, 1985.
Box 25, Folder 13
Computation Notebook #1, .
Box 25, Folder 14
Computation Notebook #2, .
Box 25, Folder 15
Computation Notebook #3, .
Box 25, Folder 16
Computation Notebook #4, .
Box 25, Folder 17
Computation Notebook #5, .
Box 25, Folder 18
Computation Notebook #6, .
Box 26, Folder 1
Computation Notebook #7, .
Box 26, Folder 2
Computation Notebook #8, .
Box 26, Folder 3
Computation Notebook #9, .
Box 26, Folder 4
Computation Notebook #10, .
Box 26, Folder 5
Computation Notebook #11, .
Box 26, Folder 6
Computation Notebook #12, .
Box 26, Folder 7
Deoxynucleotide Synthesis, Phosphonylations, Monomer Synthesis, Dimer Synthesis, .
Box 26, Folder 8
DNA Sequencing 3:728-3D, 1985.
Box 26, Folder 9
D.T. Peptide Synthesis, .
Box 26, Folder 10
Evans, Barbara - Untitled notebook, .
Box 26, Folder 14
Lamprey Transferrin (Part I), .
Additional Note
Barbara Evans, Summer 1983.
Box 26, Folder 15
Lamprey Transferrin (Part II), .
Additional Note
Barbara Evans, Summer 1983.
Box 26, Folder 16
Lamprey Transferrin: DNA Sequencing 463-1B, 1985 -1986.
Additional Note
Barbara Evans.
Box 26, Folder 17
Lamprey Transferrin: Plasmid Preps, 1984.
Additional Note
Barbara Evans.
Box 26, Folder 18
Lamprey Transferrin: Sequence 1 Molecular Biology, 1984 -1985.
Additional Note
Barbara Evans.
Box 26, Folder 19
Lamprey Transferrin: Sequencing 2: 613-3B, 463-1B, 1985.
Additional Note
Barbara Evans.
Box 26, Folder 20
Nucleotide Synthesis - Ologonucleotide Probes, 1982.
Additional Note
Barbara Evans.
Box 26, Folder 21
Peptide Synthesis - James Kobut/R. Doolittle, .
Box 27, Folder 5
AAA of Gamma Chains Trp Det, .
Box 27, Folder 6
Bead Data SP Digest of PLI (Part I), .
Box 27, Folder 7
Bead Data SP Digest of PLI (Part II), .
Box 27, Folder 8
Bead Data SP Digest PLI (Part II continues), .
Box 27, Folder 11
CN I, PL I, SP I, PL II, Tryptic Data, Chymo, SP Peptides, 1979.
Box 27, Folder 12
CN - V B, C, and D, .
Additional Note
B Gamma CN from pH2, SC -pH 9.5, CN5B -pH 9.5, SD -pH 4.5.
Box 27, Folder 13
CNBr, Cyanogen Bremide G-75 Runs, .
Box 27, Folder 14
Cys from PL2, SP PLI Parp #6, SP Digest CNI #3, SP Digest CNI #4 (Part I), .
Box 27, Folder 15
Cys from PL2, SP PLI Parp #6, SP Digest CNI #3, SP Digest CNI #4 (Part II), .
Box 27, Folder 16
D1 Binder D1 Non Binder 12/80 R and A Trypsin, .
Box 28, Folder 3
H Alpha 2, CNI, TFA, CN IB, Trypsin, part 1, .
Box 28, Folder 4
H Alpha 2, CNI, TFA, CN IB, Trypsin, part 2, .
Box 28, Folder 5
H2 CN I PL I Se #2, S rho I TR - G-25, part 1, .
Box 28, Folder 6
H2 CN I PL I Se #2, S rho I TR - G-25, part 2, .
Box 28, Folder 7
H Alpha CNI CNBr = 70% TFA, H0CNI Pl I Glu, Pl 2 Glu, .
Box 29, Folder 3
H Gamma Chain CN IV, 1975, .
Box 29, Folder 4
H Alpha CN CN -VII, 1977 (1 of 2), .
Box 29, Folder 5
H Gamma Chain CN V-VII, 1977 (2 of 2), .
Box 29, Folder 6
HD R and A BC 1980 D1 Gamma D3 Gamma Trypsin, .
Box 29, Folder 10
Gamma Chains Preps CN III, CNIV, .
Box 29, Folder 11
HXLF CEE 7177 CN I Tal 23, .
Box 29, Folder 12
Machine H Alpha CN III "G7B", 1976, .
Box 29, Folder 13
Mellitin Sequence Run, 07/18/80, .
Box 29, Folder 14
Sequence Guide 1969-70, .
Box 30, Folder 1
SP Digest #4 PLI, TR Digest Over G.50, part 1, .
Box 30, Folder 2
SP Digest #4 PLI, TR Digest Over G.50, part 2, .
Box 30, Folder 3
SP Digest CNI #6, SP Digest CNI #9 (8/79), SP Digest CNI #11 (4/79), .
Box 30, Folder 4
SP of PLI #3, SP of PLI #5, .
Additional Note
11/15/78.
High Performance Liquid Chromotography Charts
Box 31, Folder 4
Acylase, N-Ac-Ab-C-Sar, .
Box 31, Folder 11
HPLC Reverse Phase, March-April 1976, .
Box 31, Folder 12, Map-case Oversize: AB-000-01
